Evaluation of Noopept Effect on the Neurotransmitter Amino Acids in the Hippocampus in Alcohol Drinking Rats Using in Vivo Microdialysis

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The aim of the present work was to study the dynamics of neurotransmitter amino acids after acute Noopept (a dipeptide analogue of piracetam used in clinical practice as a nootropic agent) administration in intact and long-term ethanol (ETOH) exposed rats. Albino male rats were given 10% (vol/vol) ETOH solution as the only source of fluid 24 h / 7 days per week (n = 5). Also we used intact rats of the same age which had no access to ethanol (n = 5). The excitatory and inhibitory amino acids in the extracellular space of the dorsal hippocampus region in freely moving intact and ETOH-exposed rats during prolonged alcohol deprivation were measured using the intracerebral microdialysis method followed by HPLC/ED. There were no significant differences in the level of neurotransmitter amino acids between ETOH-exposed and intact animals. For the first time, in vivo experiments the effect of Noopept (1.5 mg/kg, i.p.) on the level of excitatory amino acids (an increase in ASP by 2.38 times and GLU by 2.28 times) along with an increase in the level of the inhibitory amino acid GLI by 3.13 times only in intact rats was shown. Thus, in ETOH-exposed rats under the adaptive rearrangements in prolonged ethanol withdrawal, the neurochemical mechanisms of the hippocampus seem to be characterized by insensitivity to an acute Noopept administration. Animal neurochemical studies of changes in the mediator amino acids due to the long-term effect of alcohol on the CNS may be of practical importance for the development of optimal strategies and pharmacotherapy.

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作者简介

V. Kudrin

Federal Research Center for Original and Prospective Biomedical and Pharmaceutical Technologies

Email: lgkolik@mail.ru
俄罗斯联邦, Moscow

V. Konkov

Federal Research Center for Original and Prospective Biomedical and Pharmaceutical Technologies

Email: lgkolik@mail.ru
俄罗斯联邦, Moscow

E. Shubenina

Federal Research Center for Original and Prospective Biomedical and Pharmaceutical Technologies

Email: lgkolik@mail.ru
俄罗斯联邦, Moscow

K. Kasabov

Federal Research Center for Original and Prospective Biomedical and Pharmaceutical Technologies; I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: lgkolik@mail.ru
俄罗斯联邦, Moscow; Moscow

D. Sadovnik

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: lgkolik@mail.ru
俄罗斯联邦, Moscow

A. Khairetdinova

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: lgkolik@mail.ru
俄罗斯联邦, Moscow

A. Umriyukhin

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: lgkolik@mail.ru
俄罗斯联邦, Moscow

L. Kolik

Federal Research Center for Original and Prospective Biomedical and Pharmaceutical Technologies

编辑信件的主要联系方式.
Email: lgkolik@mail.ru
俄罗斯联邦, Moscow

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2. Fig. 1. Design of the experiment.

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3. Fig. 2. The layout of the microdialysis probe in the dorsal hippocampus (a) and a photo of a section of the rat brain (b). The arrow indicates the place of origin and the direction of the trace channel of the microdialysis probe.

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4. Fig. 3. The effect of a single systemic administration of noopept on the content of aspartate (ASP) in the hippocampus of mongrel rats. *p < 0.05 compared to the level at initial rest; #p < 0.05 compared to intact rats, according to the ANOVA planned comparison analysis followed by multiple Newman—Keils analysis.

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5. Fig. 4. The effect of a single systemic administration of noopept on the content of glutamate (GLU) in the hippocampus of mongrel rats. *p < 0.05 compared to the level at initial rest, according to the ANOVA planned comparison analysis followed by multiple Newman—Keils analysis.

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6. Fig. 5. The effect of noopept with a single systemic administration on the content of glycine (GLI) in the hippocampus of mongrel rats. *p < 0.05 compared to the level at initial rest; #p < 0.05 compared to intact rats, according to the ANOVA planned comparison analysis followed by multiple Newman—Keils analysis.

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7. Fig. 6. The effect of noopept with a single systemic administration on the content of taurine (TAU) in the hippocampus in mongrel rats.

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8. Fig. 7. The effect of noopept with a single systemic administration on the content of GABA in the hippocampus in mongrel rats.

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