Development of Quantitative Enzyme Immunoassay of Brain-Derived Neurotrophic Factor on the Basis of Recombinant Antigen

O. V. Pavlova; Павлова О. В.; A. A. Murashko; Мурашко А. А.; N. V. Andriushchenko; Андрющенко Н. В.; O. I. Gurina; Гурина О. И.; K. A. Pavlov; Павлов К. А.

doi:10.31857/S1027813323010156

Development of Quantitative Enzyme Immunoassay of Brain-Derived Neurotrophic Factor on the Basis of Recombinant Antigen

作者: Pavlova O.V.¹, Murashko A.A.², Andriushchenko N.V.¹, Gurina O.I.¹, Pavlov K.A.¹
隶属关系:
1. Serbsky National Medical Research Centre for Psychiatry and Narcology
2. Moscow Research Institute of Psychiatry – the Branch of Serbsky National Medical Research Centre for Psychiatry and Narcology
期: 卷 40, 编号 1 (2023)
页面: 86-91
栏目: МЕТОДЫ
URL: https://cardiosomatics.orscience.ru/1027-8133/article/view/653960
DOI: https://doi.org/10.31857/S1027813323010156
EDN: https://elibrary.ru/EQWZUP
ID: 653960

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详细

One of the pathophysiological mechanisms of the development of some mental diseases, including depressive disorders, is a decrease in the expression of brain-derived neurotrophic factor (BDNF), mainly in the limbic region and prefrontal cortex of the brain. Immunochemical screening of BDNF in biological fluids showed that the levels of this protein can be considered as a marker of predisposition to depression and a prognostic marker of the effectiveness of therapy. The use of modern technologies for the production of recombinant proteins makes it possible to develop highly standardized ELISA systems for the determination of this antigen in biological fluids. This paper describes a method for creating a test system for quantitative ELISA of brain-derived neurotrophic factor on the basis of recombinant protein BDNF and antibodies obtained as a result of immunization with recombinant BDNF.

关键词

brain-derived neurotrophic factor, monoclonal antibodies, recombinant protein, ELISA, depressive disorders

参考

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