Mechanism of effect of the zinc and lead ions on state of the oxidation processes in liposomes from lecithin

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Abstract

The influence of divalent zinc and lead ions in a wide range of concentrations on the ability of soy lecithin to spontaneous aggregation in water medium, the zeta potential of he formed liposomes, the ability of metal ions to interact with membranes and their participation in the processes of the lipid peroxidation were studied using the method of dynamic light scattering and mathematical processing of UV-spectra of lecithin and its mixtures with metal ions. It has been shown that the scale and direction of the impact of zinc and lead ions corresponds to their biological activity when entering the body. The data obtained and the analysis of the literature allow us to conclude that the effect of zinc ions at high concentrations on the structural state of membranes and their electrophoretic properties and a significant change in the parameters of the lipid peroxidation regulation system in biological objects in the presence of lead ions, even at low doses, are the basis of their toxicity for biological objects.

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About the authors

A. V. Mashukova

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: shishkina@sky.chph.ras.ru
Russian Federation, Moscow

A. S. Dubovik

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences; Nesmeyanov Institute of Organoelement compounds, Russian Academy of Sciences

Email: shishkina@sky.chph.ras.ru
Russian Federation, Moscow; Moscow

V. O. Shvydkiy

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: shishkina@sky.chph.ras.ru
Russian Federation, Moscow

L. N. Shishkina

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Author for correspondence.
Email: shishkina@sky.chph.ras.ru
Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Dependence of the ζ-potential values ​​of lecithin liposomes ([Lecithin] = 4.3 10–5 M) on the concentration of lead ions (1, lecithin batch No. 2; 2, lecithin batch No. 1) and zinc ions (3, lecithin batch No. 1).

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3. Fig. 2. Ultraviolet spectrum of lecithin in the presence of zinc ions and its Gaussians: 1 and 2 – initial and calculated spectra, 3 – 196.4 nm, 4 – 231.0 nm, 5 – 265.8 nm, 6 – 343.6 nm, 7 – 407.7 nm; [Lecithin] = 4.3 10–5 M, [Zn]2+ = 5 10–5 M.

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4. Fig. 3. Ultraviolet spectrum of lecithin in the presence of lead ions and its Gaussians: 1 and 2 – initial and calculated spectra, 3 – 198.7 nm, 4 – 209.6 nm, 5 – 232.4 nm, 6 – 260.8 nm, 7 – 352.6 nm; [Lecithin] = 4.3 10–5 M, [Pb]2+ = 5 10–5 M.

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5. Fig. 4. The ratio of the content of ketodienes (KD) and diene conjugates (DC) in liposome lipids depending on the concentration of zinc (1) and lead (2) ions in the solution.

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