Molecular epidemiological analysis of HIV-1 genetic variants circulating in the countries of Eastern European and Central Asia in 2010-2019


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Abstract

Objective. To characterize HIV-1 genetic variants circulating in the countries of Eastern Europe and Central Asia (EECA) in 2010-2019. Materials and methods. Nucleotide sequences of the HIV-1 pol gene fragment (2253-3344 bp) in the plasma samples were obtained from HIV-infected patients in Russia (RU), Armenia (AM), Azerbaijan (AZ), Belarus (BY), Kyrgyzstan (KG), Tajikistan (TJ), and Uzbekistan (UZ). The collection was supplemented with nucleotide sequences from the international database. Results. A total of1825nucleotide sequences from the countries studied were analyzed. Infection through unprotected heterosexual contact was the dominant route of transmission (about 50%). There was a decrease in the proportion of injecting drug users in 2010-2019. The dominant HIV-1 genetic variants were subtype A6 (64.82%), CRF63 02A1 (22.58%), and subtype B (7.40%). Moreover, the share of CRF63 02A1 in Central Asian countries was significantly higher than that in other EECA countries. There were 48 unique recombinant forms, 38 of which were AG recombinants. 140 molecular clusters were formed containing 369 (20.22%) sequences. Conclusion. There is a need for further investigations of HIV-1 genetic diversity in the EECA region and for supervision over the generation and spread of new HIV-1 recombinant forms.

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About the authors

Ilya A. Lapovok

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human WellBeing

Email: i_lapovok@mail.ru
Cand. Biol. Sci., Senior Researcher, Central Research Institute of Epidemiology

Alina A. Kirichenko

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human WellBeing

Email: kotova-kirichenko@mail.ru
Researcher

Anastasia V. Shlykova

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human WellBeing

Email: murzakova_a.v@mail.ru
Researcher, Central Research Institute of Epidemiology

Daria V. Saleeva

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human WellBeing

Email: dasha_saleeva@inbox.ru
Researcher, Central Research Institute of Epidemiology

Alexey E. Lopatukhin

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human WellBeing

Email: a.lopatukhin@gmail.com
Researcher

Dmitry E. Kireev

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human WellBeing

Email: dmitkireev@yandex.ru
Cand. Biol. Sci., Head, Laboratory

Natalya N. Ladnaia

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human WellBeing

Email: n.ladnaya@hiv-russia.ru
Cand. Biol. Sci., Senior Researcher

Erkin I. Musabaev

Research Institute of Virology, Tashkent

MD, Director

Evgeniya I. Kazakova

Research Institute of Virology, Tashkent

Cand. Med. Sci., Junior Researcher

Nargiz S. Ibadullaeva

Research Institute of Virology, Tashkent

Cand. Med. Sci., Senior Researcher

Sabina S. Ibragimova

Republic Center for AIDS Control, Baku

Email: cascabell@mail.ru
Laboratory Doctor

Aygun M. Safarova

Republic Center for AIDS Control, Baku

Email: aygun.seferova88@gmail.com
Laboratory Doctor

Agigat Kadirova

Research Institute of Pulmonary Diseases, Baku

Email: office@aids.az
Director

Zukhra Nurlyaminova

Republican Center for AIDS Prevention and Control, Dushanbe

Email: zuha_69@bk.ru
Deputy Director

Lailo Ismatova

Republican Center for AIDS Prevention and Control, Dushanbe

Email: l-ismatova@mail.ru
Head, Reference Laboratory

Mohira Zukhurova

Republican Center for AIDS Prevention and Control, Dushanbe

Email: mz-87-87@mail.ru
Laboratory Doctor

Ramshed Kholnazarov

Republican Center for AIDS Prevention and Control, Dushanbe

Email: rholnazarov@mail.ru
Infectiologist

Aibek A. Bekbolotov

Republican AIDS Center, Ministry of Health of the Kyrgyz Republic

Email: aibek_0001@mail.ru
Deputy Director

Elmira B. Narmatova

Osh Regional AIDS Center, Ministry of Health of the Kyrgyz Republic

Email: elmira.narmatova@yandex.ru
Chief Physician

Baarinisa M. Iskanova

Republican AIDS Center, Ministry of Health of the Kyrgyz Republic

Email: i.barnisa@hotmail.com
Laboratory Assistant

Trdat R. Grigoryan

Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia

Email: gtrdat@yahoo.com
Monitoring and Evaluation Specialist

Arshak R. Petrosyan

Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia

Email: petrosyan@list.ru
Laboratory Doctor, Laboratory of PCR, Microbiology, Virology and Genetics

Tatevik A. Sargatyan

Republican Center for AIDS Prevention, Ministry of Health of the Republic of Armenia

Email: sarhatyantatev@mail.ru
Laboratory Doctor, Laboratories of PCR, Microbiology, Virology, and Genetics

Elena L. Gasich

Republican Research and Practical Center for Epidemiology and Microbiology

Email: gasich@gmail.com
Cand. Biol. Sci., Associate Professor, Head, Laboratory Minsk, Belarus

Anastasia S. Bunas

Republican Research and Practical Center for Epidemiology and Microbiology

Email: rrpcem@belriem.by
Junior Researcher Minsk, Belarus

Iryna N. Glinskaya

Republican Center for Hygiene, Epidemiology, and Public Health

Email: mail@rcheph.by
Epidemiologist, Republican Center for Hygiene Minsk, Belarus

Vadim V. Pokrovsky

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Right Protection and Human WellBeing

Email: pokrovsky.vad@yandex.ru
Academician of the Russian Academy of Sciences, MD, Head, HIV Department

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